KMID : 1025520000420010009
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Journal of Animal Science and Technology 2000 Volume.42 No. 1 p.9 ~ p.20
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Characterization of Lactoferrin Gene 5¡¯ Flanking Region of Korean Native Goat
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Yu S. L.
Lee T. H. Sang Byung-Chan Shin Sang-Tae Lee Kyung-Kwang Yu Dae-Yeul
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Abstract
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Lactoferrin(Lf), a ferric ion(Fe3+)-binding glycoprotein, is found most notably in milk and some exocrine secretions. It is a main antimicrobial component of colostrum and milk that contributes to protection of the infant against infectious diseases. To analyze 5¡¯flanking region of Korean native goat lactoferrin gene, we cloned and sequenced 975 bp of 5¡¯flanking region of Korean native goat Lf gene. When compared to those of bovine and human Lf, the nucleotide sequences exhibited about 90.9% and 49.7% homology, respectively. The transcription start site was localized by primer extension to residue C, which is 38 nucleotides upstream from the ATG start codon. The 5¡¯flanking region of Korean native goat lactoferrin gene contains putative transcription factor binding sites such as SP1, CAT, C/EBP and GATA-1.
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KEYWORD
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Korean native goat, Lactoferrin, 5¡¯Flanking region, Transcription factor(SP1, CAT, C/EBP and GATA-1)
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